Isomerase has acquired and generated a large (>20,000 strains) eukaryotic and prokaryotic microbial strain collection. This is managed via a carefully curated corporate relational database that links the strains to data generated using the strains and the compounds they are known to produce and has originated from a variety of sources, including Biotica Technology Ltd., Cambridge University, Bath University, and a variety of biotechs such as Zuvasyntha. It includes a biotransformation collection (>1500 strains), a novel actinomycete collection of >7000 strains, an extremophile collection (>2500 strains) along with additional strains from publicly available patent collections, strain collections and engineered generated in-house at Isomerase, such as our collection of engineering Pichia pastoris and Escherichia coli protein expression strains. These strains include numerous chassis strains with extensive industrial heritage and both novel and published strains, including talented strains (i.e. those capable of producing a wide variety of products). The collection is Nagoya-compliant and available for use on partner projects under suitable terms.
Genome sequence library
Isomerase has expertise in generating genomic DNA from a range of microbial and other sources and a network of preferred partners for raw sequence generation. We have in-house software, pipelines, and expertise for the assembly of genome sequences and for their bioinformatic analysis. This can be used, for example, to genome sequence novel strains, and then identify enzyme activities, biosynthetic gene clusters and other activities and pathways of interest.
This strain collection includes many actinomycetes, which are known as powerhouses of biosynthesis and catabolism. This proprietary database of genomes is a hugely valuable resource to identify enzymes for biotransformation and biocatalysis, pathways for production of a large variety of products, biosynthetic gene clusters for heterologous expression and targeted manipulation and enzyme domains for use in targeted non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) engineering and rapid access to homologous clusters and potential analogues of products of interest.
Please contact us for further information about our microbial strain collection, our biotransformation collection, our extremophiles, such as thermophiles and psychrophiles or our actinomycete collection.