The ability to rapidly read/write and assemble DNA means we can access the genetic information for natural products from almost any microbial or plant source. Isomerase has developed, and is continuing to generate an extensive proprietary synthetic biology toolkit which allows us to construct and assemble genes into novel functional pathways and express them in suitable hosts.
To enable this, we have a suite of host organisms, including fungi, Gram-positive and -negative bacteria, and those based on industrial scale multi-gram natural product producers, each with a suite of vectors and methods which enable facile manipulation and regeneration of pathways.
Examples of where we can have impact include:
- Heterologous Expression - Express new or improved pathways in a range of heterologous hosts and industrial production strains
- Analogues - biosynthetic manipulation to generate analogues around a known template
- Pathway Construction - reconstruction/manipulation of biosynthetic pathways from difficult-to-access organisms to access to new chemistry
- Biocatalysis - expressing and isolating biocatalytic enzymes from eukaryotic and prokaryotic organisms
- Protein Manipulation - generating and testing diverse variants of target proteins, for example through manipulation of substrate specificity