Isomerase were approached by a chemicals manufacturer looking to generate alternative processes to a range of enzymes for biocatalysis.

• Solution: Development of an enzyme secretion system to allow direct lyophilization of fermentation supernatants

We generated a panel of microbial expression strains with vectors containing a secretion tag. Fermentation was carried out to confirm presence and activity in the broth with cellular material removed. A DSP method was then developed which enabled lyophilised enzyme to be isolated directly from the fermentation broth.

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