Synthetic Biology and Bioengineering

Isomerase has developed, and is continuing to generate, new tools and methods for the synthetic generation and manipulation of genetically encoded microbial pathways. These can be used in a variety of applications, including:

• Rebuilding and then manipulating biosynthetic pathways from difficult-to-access organisms, enabling access to new diversity and lowering cost of goods.

• Expressing and isolating biocatalytic enzymes from eukaryotic and prokaryotic organisms.

• Generating and testing diverse variants of target proteins, for example through manipulation of substrate specificity.

To enable this we have a suite of host organisms, including Gram-positive and -negative bacteria and those based on industrial scale multi-gram natural product producers, each with a suite of vectors and methods which enable facile manipulation and regeneration of pathways.